Answer the following questions about CRISPR/Cas9 using the information provided in class: a) What is the natural function of CRISRP/Cas and where is it found? (1 pt) b) Annotate the following diagram showing the engineered CRISPR/Cas9 system by filling in the missing information for the numbers in the table below (4 pts)    Answer 1: 2: 3: 4: 5: 6: 7: 8:   c) Explain how the engineered CRISPR/Cas9 system differs from the natural system. Specifically, how the natural system was adapted for engineering purposes and what had to be done to get this bacterial system to function in eukaryotic cells? (3 pts) d) CRISPR/Cas9 typically generates short insertions and deletions at a target site via which repair target pathway in eukaryotic cells? (1 pt) e) Which mechanism would you need to exploit to achieve site-specific DNA insertion into a CRISRPR/Cas9 target site? Explain how this would work for replacing e.g. a gene or introducing a mutation. Can you think of a strategy to increase the efficiency that this will happen? (2 pts) f) What needs to be present in a bacterial cell for efficient DNA/gene insertion using CRISPR/Cas9? (1 pt) g) What does dCas9 stand for and give three different examples on how dCas9 is used? (3 pts)

A patient presents to the Emergency Department after a sudden gust of wind blew a pile of sawdust into his eye while working outdoors with a saw. The nurse explains to the patient that what treatment in likely indicated?

What advantage is there to starting Sandy on enteral nutrition shortly after arriving in the ICU?     Patient Scenario: Sandy is a 66-year old female admitted to the ICU with respiratory failure .  She is currently sedated, on a mechanical ventilator and receiving the antibiotic azithromycin.  Ht: 165 cm          Wt: 73 kg            Temp: 39.3°C          Ve: 12 L/min                 Propofol: 10 ml/hr     Penn State 2010: Mifflin (0.71) + T max C˚(85) + Ve (64) – 3085    

Multiple Choice Select the correct answer for each question (40 questions, 1 point each).

What is Ella’s weight gain goal? Patient scenario: Ella is a 980 gram female infant born at 27 weeks gestation.  She is 36 cm in length and her head circumference was 25 cm.     

How many ml of fluid is Sandy receiving from her tube feeding? Sandy has an NG tube placed and is receiving 45 ml/hr of Pivot.  Pivot contains:   1.5 cal/ml            93.8 g protein/L                759 ml water/L.  

Which of the following four text options is correct? Type the corresponding number into the box below. Production of industrially important aromatic amino acids    1   mostly leads to phenylalanine, alanine, and tryptophan    2   can be increased by optimizing the Shikimate pathway    3   is not dependent on the central carbon metabolism    4   is dependent on a membrane bound PQQ-dependent alcohol dehydrogenase

In a confusion matrix for a customer churn model, where the positive class is Churn and the negative class is Did Not Churn, what does FN (False Negative) represent?

Bonus Question: 1st step:  Offer a paragraph that details your favorite topic from the class.  (5 points) 2nd Step:  What is qualified immunity (1 point)?   What constitutes the protection it offers (2 points)and why would an officer not receive protection (2 points).   (5 points possible)  

Researchers are trying to understand whether phosphorus limits crop productivity in wheat.  They set up plots with no doses of 0, 25, 50 75 and 100.  They then examine the change in wheat productivity (measured as yield in kg/hectare).  Their results are shown below in table and in graph form. Based on this, which of the following is accurate? (select all that apply) Phosphorus fertilizer dose yield (kg/ha-1) 0 7 25 23 50 33 75 32 100 33