When would a transfer (Pasteur) pipette be used in the lab?

You have a stock of DNA ligase at 50,000 units/µl. You need 5,000 units of ligase in a 25 µl reaction, how many microliters of ligase do you need?

How do you prepare 600 ml of 50 mM Tris (Mw = 121.14)?

You add 1000 microliters of Folin reagent to a lysate sample and read its absorbance in the spectrophotometer, which gives a reading of 0.3 compared to a blank. This is a(n) ________ measurement.

Which type of inhibitor greatly reduces the Vmax of a reaction, and can’t be overwhelmed by substrate?

You wish to study the effects of substrate concentration on enzyme rate. For the experiment, you alter the substrate concentration and measure the change in enzyme rate, while making sure the enzyme concentration is constant in each sample. Which is the control?

A published clinical study shows a drug is ineffective against a disease, but doctors continue to prescribe it due to some of their patients reporting that the drug is working. This is an example of 

A type of centrifugation used in the lab to collect large volumes (e.g. 1 L bottles) of bacterial cells is

You add some Folin reagent to a lysate sample, and it turns slightly blue. This is a(n) ________ measurement.

Amino acids differ due to their unique