If you perform a genetic experiment in E. coli such that you replace the bacterial RNAP with yeast RNA Pol II, which of the following would you most likely find?

You are fascinated by the biochemical pathway that leads to the biosynthesis of the amino acid Arginine that we learned about in the Beadle Tatum experiment. You mutagenized E. coli, and identified an Arginine auxotroph in which the mutation maps to Gene 3 encoding the Enzyme 3 that converts Citrulline to Arginine. You find the Gene 3 mutation resulted in a deletion of an inverted repeat sequence occurring 5′ (upstream) to a GT-rich sequence at the 3′ end of the gene.  Further examination shows that the gene is able to be transcribed but no protein is made (hence it being an auxotroph for Arginine). Based on these data, your most likely prediction would be:

You are studying a yeast mutant where the CTD tail of RNA Pol II has several key serines (phosphorylatable amino acids) converted to alanines (non-phosphorylatable). The result of these changes is the failure of polyA polymerase (PAP) to bind during transcription. The resulting RNA polymerase protein would most likely  be capable of synthesizing:

Which of the following are functions of mediator complexes? (select all that apply)

You are fascinated by the biochemical pathway that leads to the biosynthesis of the amino acid Arginine that we learned about in the Beadle Tatum experiment. You mutagenized E. coli, and identified an Arginine auxotroph in which the mutation maps to Gene 3 encoding the Enzyme 3 that converts Citrulline to Arginine. You find the Gene 3 mutation resulted in a deletion of an inverted repeat sequence occurring 5′ (upstream) to a GT-rich sequence at the 3′ end of the gene.  Further examination shows that the gene is able to be transcribed but no protein is made (hence it being an auxotroph for Arginine). Based on these data, your most likely prediction would be:

In between the first and second transesterification reactions of RNA splicing, the intron of the pre-mRNA

The mediator complex components differ between organisms but otherwise stay the same in each cell type

What is the nucleophile of the first splicing transesterification reaction?

An RNA processing event that frequently leads to different protein products encoded by a single gene is:

The chemical structure of the 5’ mRNA cap includes __ covalently linked to the 5’ terminal residue of the mRNA through a __ linkage.