Which ion has the largest radius?                   Ca+2, Ca+1, Br-, K+ 

A gold nugget (mass = 376 g) is originally at 398 K is placed in cold water. Its temperature drops to 297 K. If the specific heat capacity of gold is 0.128 J/g°C, how much heat was lost by the gold nugget? Must show your work (scratch paper) to receive credit.

Phosphorylation of which protein(s)’s C-terminal domain tail serves as the timer and spatial organizer of eukaryotic transcription?

You are studying a yeast mutant where the CTD tail of RNA Pol II has several key serines (phosphorylatable amino acids) converted to alanines (non-phosphorylatable). The result of these changes is the failure of polyA polymerase (PAP) to bind during transcription. The resulting RNA polymerase protein would most likely  be capable of synthesizing:

You have identified a new Sigma factor that can bind directly (with varying affinities) to DNA regions that have the sequences shown below. Based on these results, what is the strongest promoter sequence?  

If you perform a genetic experiment in E. coli such that you replace the bacterial RNAP with yeast RNA Pol II, which of the following would you most likely find?

You are fascinated by the biochemical pathway that leads to the biosynthesis of the amino acid Arginine that we learned about in the Beadle Tatum experiment. You mutagenized E. coli, and identified an Arginine auxotroph in which the mutation maps to Gene 3 encoding the Enzyme 3 that converts Citrulline to Arginine. You find the Gene 3 mutation resulted in a deletion of an inverted repeat sequence occurring 5′ (upstream) to a GT-rich sequence at the 3′ end of the gene.  Further examination shows that the gene is able to be transcribed but no protein is made (hence it being an auxotroph for Arginine). Based on these data, your most likely prediction would be:

You are studying a yeast mutant where the CTD tail of RNA Pol II has several key serines (phosphorylatable amino acids) converted to alanines (non-phosphorylatable). The result of these changes is the failure of polyA polymerase (PAP) to bind during transcription. The resulting RNA polymerase protein would most likely  be capable of synthesizing:

Which of the following are functions of mediator complexes? (select all that apply)

You are fascinated by the biochemical pathway that leads to the biosynthesis of the amino acid Arginine that we learned about in the Beadle Tatum experiment. You mutagenized E. coli, and identified an Arginine auxotroph in which the mutation maps to Gene 3 encoding the Enzyme 3 that converts Citrulline to Arginine. You find the Gene 3 mutation resulted in a deletion of an inverted repeat sequence occurring 5′ (upstream) to a GT-rich sequence at the 3′ end of the gene.  Further examination shows that the gene is able to be transcribed but no protein is made (hence it being an auxotroph for Arginine). Based on these data, your most likely prediction would be: