You successfully complete the cobra venom project and are ready to make the ointment…. The remaining questions deal directly with our own MCB4034L experiments.

What was the rationale for using as E. coli expression strain BL21 (DE3) pLysS? Select the correct answer from the choices below.

Select from the answers below why proteins are transferred from a polyacrylamide gel to a membrane.

Momentum Automobiles, an automobile manufacturer, hires Dreams Inc. to cater to its advertising needs. Momentum is only required to provide the necessary finances and does not take part in any decision making. In the given scenario, Momentum Automobiles would be classified as a(n)

The image associated with this question shows a stained polyacrylamide gel following electrophoresis.  The lane numbers are placed at the bottom of the image. Which of these lanes (may be none, one, or more than one) represent(s) ‘apparent homogeneity’?

Identify the principal reason the German’s decided to reinstate their strategy of unrestricted submarine warfare in 1917 in spite of the United States’ warnings doing so would lead to America’s entry into the Great War.

The functional assay we performed on the last day of class included incubation of the GFP-CBM protein with both starch and cellulose. What was the reason we included both of these polymers instead of just one?

You are getting ready for the PCR. Select from the choices below all of the ingredients (i.e. multiple answers may be correct) you will need (while ignoring the ones you don’t need) in a polymerase chain reaction to amplify a DNA fragment using as your template genomic DNA from the king cobra.

As you learned throughout MCB4034L, there are often pros and cons associated with each procedure.  Referring back to the previous question, even though the sensitivity is greater when using two antibodies, using just one antibody can be beneficial, assuming there is enough protein on the membrane.  Select the correct reason(s) from the choices below. There may be more than one correct answer.   

The blocking buffer used as part of your western blot experiment contains non-fat milk powder purchased at a local supermarket. This is an inexpensive solution to obtaining which ingredient responsible for the blocking?