You are conducting an epigenetic study and you wish to analyze the methylation of DNA at a specific site in the genome. Which of the following techniques should you use?  

In Sanger sequencing,

In nested PCR,

In the RNase protection assay,

A method to detect RNA that involves RNA digestion is

You are working with a vector that has both an ampicillin and kanamycin resistance gene. If you insert your gene into the ampicillin gene, the colonies will

If the annealing temperature in PCR is 5 °C above the Tm of the primers,

If half of E. coli cells take up a vector, and the other half the vector with insert, which of the following is TRUE?

Which technique involves cutting up DNA, ligating adapters to each end, and using primers to PCR amplify from the adapters to analyze differences between individuals?

Which technique involves attaching primers to a bead under oil, which allows the PCR product to essentially walk along the bead for continued amplification?