You wish to delete an entire protein domain. What is a practical approach to this?

Which of the following is FALSE regarding Taq and Pfu DNA polymerases?

Which technique would be useful in screening a patient sample for thousands of different viruses at a time?

In TOPO TA cloning,

Which of the following describes a standard PCR reaction, in order?

Which sequencing method involves detecting the nucleotide directly, without halting synthesis or digesting fragments?

If the annealing temperature in PCR is 5 °C below the Tm of the primers,

You are interested in performing blue and white screening. You plate cells containing a vector with no insert in the presence of ampicillin and X-gal (no IPTG). Which of the following would you expect to observe?

To verify the sequence of an insert in a vector with Sanger sequencing, you must use

You perform Sanger sequencing on a plasmid which came from an E. coli colony. At one position you find two different overlapping peaks at 50% of the height of surrounding peaks, which are all identical to one another. What can you conclude from this?