You are developing an experiment to determining the quantity of DNA target molecules in a sample. Which of the three PCR techniques (“Regular” PCR, RT-PCR, qPCR) would be most appropriate for this?

If you think about the entire process of gene expression, from DNA to protein, you could argue that the actual step of “translation” is when

True/False regarding bacterial transcription. All of the promoter sequences are the same for all of the genes in any one species in the Domain Bacteria.

Comparing and contrasting RNA polymerase that makes RNA and DNA polymerase that makes DNA , identify which statements are true (accurate) and which are false (inaccurate). This is a multiple answer question. 

If the following piece of double-stranded DNA is transcribed, using the TOP strand as the template strand, what would be the RNA molecule produced?   5’TCCGCAACACTGT3′ 3’AGGCGTTGTGACA5′

In Bacteria and Archaea, the Ribosome Binding Site is [blank1] the start codon. The first amino acid in the protein is specified by [blank2]. 

Bacterial RNA [answer1] all have a ribosome binding site. This [answer2] true of Archaea. By contrast, in general  [answer3] of eukaryal nuclear transcripts have a ribosome binding site. 

True/False. Termination of translation is signaled by the binding of release factors to the E site.

During DNA replication, there is typically [answer1] DNA polymerase complexes at the replication fork and [answer2]  helicase complexes. 

True/False. Upon peptide bond formation, an amino acid or peptide is transferred from a tRNA in the P site to an amino acyl-tRNA in the A site.