What is the minimum passing grade required for this course (based on LSBPNE guidelines)?

Enzymes are produced on a large scale for different applications. For each of the applications below choose an enzyme from the drop down menu.   In the baking industry, the following enzyme[bakingenzyme1], is used to increase dough rising. For high fructose corn syrup production, the following enzyme is the key biocatalyst to make this sweetener[HFSenzyme]. Several enzymes are used in meat processing, one enzyme in particular is now used to create processed meat products (“blocks”) from pieces of meat[meatglue] For “degumming” during juice production this enzyme[fruit] is important for preventing clogging of processing equipment. Finally, this particular enzyme[depilling]  is used in the textile industry to create the stone washed effect of jeans and in laundry detergents for de-pilling of clothes.

You have been asked to express protein X in E. coli. Upon cloning the gene encoding this protein into your favorite expression vector, you realize that you first of all have a hard time cloning (obtaining only few transformants) and that cells transformed with the plasmid (that you finally managed to make), grow very slowly and cultures never reach high cell densities.  Briefly answer the following two questions: 1. What is the most likely reason for your difficulties? 2. How can you overcome this challenge? Name three strategies that you could use to solve your problem.

What makes the industrial use of oxidoreductase enzymes more costly and how is this issue usually dealt with?

Polyketide synthases (PKS) are important biosynthetic assembly lines composed of one or more modules that together synthesize an enormous number of structurally diverse secondary metabolites. A minimal PKS module is composed of 4 essential catalytic domains that catalyze one condensation and subsequent release reaction. Answer questions a-d. a.  Name the 4 domains and catalytic activity in the order that they have to perform their functions. (2 pts) Domain 1: Domain 2: Domain 3: Domain 4: b. Name the typical starter and extender molecules that are linked together by one PKS catalyzed reaction (1 pt)   c. Name the reaction mechanism by which these two molecules are linked together. (1 pt)   d. Write down the structural formula of the final reaction product upon release from the PKS after one reaction round. (1 pt)

Which of the following four text options is correct? Type the corresponding number into the box below. Anaplerotic reaction(s) 1  are not involved in the citric acid production process 2  describe the conversion of glucose to fructose 3  are important for anaerobic fermentation processes 4  have high flux in the glutamic acid production process

You have synthesized the complete genome of B. subtilis and are now planning on transforming this synthetic genome into E. coli using the transformation methods developed for Mycoplasma. Assuming that you are able to get the genome transferred into E. coli, what problems will you encounter that would make it unlikely that you would obtain viable cells that are now reprogrammed with genetic information from B. subtilis? Come up with four potential problems and explain why they are bottlenecks for B. subtilis genome transplantation into E. coli.

Multiple Choice (7 questions @ 1 point each = 7 points). Please indicate corresponding the BEST answer.If a company with a process costing system chooses to use the weighted average method to determine the cost per equivalent unit of production, then costs associated with partially complete units in beginning work in process are:

Problem (14 Points) A manufacturing company that produces a single product has provided the following data concerning its most recent month of operations:   (a) What is the net operating income for the month under absorption costing?  (7 points) (b) What is the net operating income for the month under variable costing? (7 Points)

Gangwer Corporation produces a single product and has the following cost structure:  The absorption costing unit product cost is: