Sometimes when small molecule drugs have off-target effects, they can actually be repurposed as a drug for other diseases. Now that you have identified the protein as HSP90, you decide to conduct more experiments to characterize this new interaction between your drug and the protein. You clone the gene into an expression vector that is engineered with a GST tag. Once you express your protein, propose a workflow on how you would purify this protein. You should use your pI from question 11 of 8.6 and include a buffer exchange and two types of chromatography. If you choose an ion exchange or affinity chromatography, identify which kind you will use. 

Maria has strong political views and ignores anyone who thinks differently than she does. Maria demonstrates selective

List four factors that affect radiosensitivity in an organism and how they affect radiosensitivity.

The antiviral drug being developed is found bind to another protein in the tissues, as seen in the previous question. To further identify which protein your drug is binding to, the protein is extracted from the gel and prepared for mass spectrometry analysis. The results can be found on the mass spectra below: Using the indicated mass of 2442.4 as your M1, determine the charge (z) of that peak, then from that peak, determine the identity of your protein based on your calculated mass. Charge (z): [charge] Protein identity: [protein]

An experiment was conducted on human epithelial cells to determine the amount of radiation it would take to kill 75% of the cells. For this particular experiment it took 30 Gy of x-radiation to kill 75% of the cells. During the same experiment, it took 5 Gy of protons to kill the same percentage of cells. What is the RBE for protons? What does your answer tell us?

Below is the pKa chart you can use for your exam: Amino Acid pKa-COOH pKa-NH3 pKa-R pI Non Polar Aliphatic Glycine 2.34 9.60 5.97 Alanine 2.34 9.69 6.01 Proline 1.99 10.96 6.48 Valine 2.32 9.62 5.97 Leucine 2.36 9.60 5.98 Isoleucine 2.36 9.68 6.02 Methionine 2.28 9.21 5.74 Aromatic Phenylalanine 1.89 9.13 5.48 Tyrosine 2.20 9.11 10.07 5.66 Tryptophan 2.38 9.39 5.89 Amino Acid pKa-COOH pKa-NH3 pKa-R pI Polar Uncharged Serine 2.21 9.15 5.68 Threonine 2.11 9.62 5.87 Cysteine 1.96 10.28 8.18 5.07 Asparagine 2.02 8.80 5.41 Glutamine 2.17 9.13 5.65 Positively Charged Lysine 2.18 8.95 10.53 9.74 Histidine 1.82 9.17 6.00 7.59 Arginine 2.17 9.04 12.48 10.76 Negatively Charged Aspartic Acid 1.88 9.60 3.65 2.77 Glutamic Acid 2.19 9.67 4.25 3.22

After a Compton interaction with an outer shell electron of a calcium atom (BE = 0.04), the recoil electron sped away with 10 keV of energy. The scattered photon was deflected at an angle of 75 degrees from the original photon retaining 62 keV of energy. What was the energy of the incident photon?

R-propanolol is a synthetic drug, and therefore no isomerase exists in the body to convert it to S-propanolol.  R-propanolol in the presence of beta-receptors has a Keq of around 46500 at room temperature (25oC). What is the ΔG° of binding R-propanolol to the beta-receptor?  (R = 8.314 J/mol*K)

The Company Name Formatting program has been wrapped in a user-defined function. Assume companies is a list of names created earlier in the program. def format_companies(names):    formatted = []    for name in names:        formatted.append(name.title())    return formattedcompanies_title = format_companies(companies) Which statement about this code is correct?

Match the following methods to their descriptions