23.  What is the difference between a wet mount and Simple stain?

5. This is MacConkey agar plate divided into 2 sections.  Section A is streaked with             organism A and section B with organism B. Describe the reactions in sections A and             B and give a reason for the reactions (4pts).                         A————————————————————————————————             B————————————————————————————————-   B.        What type of media is MacConkey Agar? (2pts).               —————————————————————————————————

22.  Name 2 environmental factors that can influence the growth pattern of microorganisms. 

20.  These are SIM media.  Describe the reactions in tubes A, B, C, D, E

25. Name and describe 3types of Flagella

27.  Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture and told you to make a 10-1 dilution of the entire culture.  Explain how you would do this.  Show your calculations.

28.  You plated 0.1 mL of a sample diluted by a factor of 10-5 and after incubation you counted 50 colonies.  The concentration of bacteria in the original culture is:  NOTE: 10-5 is 10 to the negative 5 and 10+5 is 10 to the power of positive 5.

18. Give two reasons for the differential staining between gram + and Gram – organisms (2pts).

17.  We used Columbia CNA with 5% Sheep Blood media in the Lab.  This media is        both Selective and differential.  What is it differential for? (2pts)  ………………………………………………………………………………………   B.  What makes this media Selective and what is it selective for? (2pts)             ————————————————————————————————— C.   Why do you incubate plates upside down? (2pts). —————————————————————————————————–

1.      Name the labelled parts (1, 2, 3, 4, 5, 6, 7, 8) of the Light Compound Microscope shown in             the picture.  Make sure the numbers on the label correspond to the             Numbers on the answer sheet (4pts). HINT: the line after  line 4 is 5,  next is 6, skip the line after 6, the next line is 7 followed by 8.