What fluorescence pattern would you expect to see in transgenic cells that express a green fluorescent protein (GFP) tagged with a nuclear localization signal (NLS)?  

What effect does a guanine nucleotide exchange factor (GEF) have on GTPases? 

Correctly order the steps that must be taken in order for a protein to be translated in conjunction with the endoplasmic reticulum (ER): SRP = signal recognition particle 1. The ribosome dissociates from the ER membrane 2. Translation of the polypeptide through the ER pore protein (part of the translocon) into the ER lumen occurs 3. SRP binds to an SRP receptor located on the rough ER membrane 4. SRP recognizes and binds to the ER signal sequence of the newly forming polypeptide 5. An enzyme called a signal peptidase removes the ER signal sequence from the polypeptide  6. Translation of the newly forming polypeptide pauses

What is the function of heat shock protein 70 (Hsp70)? 

Your colleague wants to examine receptor-mediated endocytosis in a mutant cell line. Specifically, they want to obtain an image that is a “slice” through a cell, showing both the inside and the outside of the cell at the same time. What type of imaging technique should they use?

Glycosylation is

Proteins transported from the endoplasmic reticulum to the Golgi apparatus are carried in vesicles surrounded by:

A polypeptide with a stop-transfer sequence will be a:

What is co-translational folding?

A solute that is moved across a membrane from an area where it is highly concentrated to an area where it is less concentrated is moving: