To verify the sequence of an insert in a vector with Sanger sequencing, you must use

You perform Sanger sequencing on a plasmid which came from an E. coli colony. At one position you find two different overlapping peaks at 50% of the height of surrounding peaks, which are all identical to one another. What can you conclude from this?

In ligation independent cloning,

What primers would you design to amplify the following sequence of DNA? 5’-TTGCGTTGAGCAGTTGAGTACTTAGGCCTGCAGTGTAGAGTC-3’

100 micrograms/ml of DNA would give an A260 of

In the gel below, the DNA in Lane 4 is approximately

You use a dot blot to analyze a specific locus. Who is homozygous at this locus?

What is the advantage to introducing mutations into gene that is in already in a vector, rather than from a piece of linear DNA?

VNTR analyzes differences in DNA between individuals by comparing

You wish to insert a gene into the vector below, grow the cells on ampicillin, and select colonies with blue/white screening. Which restriction enzyme(s) should you use?