In ligation independent cloning,

What primers would you design to amplify the following sequence of DNA? 5’-TTGCGTTGAGCAGTTGAGTACTTAGGCCTGCAGTGTAGAGTC-3’

100 micrograms/ml of DNA would give an A260 of

In the gel below, the DNA in Lane 4 is approximately

You use a dot blot to analyze a specific locus. Who is homozygous at this locus?

What is the advantage to introducing mutations into gene that is in already in a vector, rather than from a piece of linear DNA?

VNTR analyzes differences in DNA between individuals by comparing

You wish to insert a gene into the vector below, grow the cells on ampicillin, and select colonies with blue/white screening. Which restriction enzyme(s) should you use?

The purity of a DNA sample is determined by looking at the

You design two primers as shown. Which of the following is TRUE? Primer 1: ATGC Primer 2: GGCT