Whаt оccurs during the terminаtiоn phаse оf translation?
11. In the secоnd step оf glycоlysis, glucose-6-phosphаte (G6P) is converted into fructose-6-phosphаte (F6P) by phosphoglucoisomerаse. 1) Circle the anomeric carbon on the above cyclic structures of G6P and F6P and indicate whether they are α- or β-form. (4 pts) 2) Draw Fischer projections of the linear form of G6P and F6P (6 pts) 3) Draw a curly arrow mechanism of the isomerization reaction catalyzed by phosphoglucoisomerase. You can simplify the acid and base in the enzyme active site as BH and B:, respectively. (20 pts) 4) Write down the relationship between the actual free energy change and the standard free energy change for this reaction AND calculate the ΔG of isomerization reaction catalyzed by phosphoglucoisomerase (ΔG˚’ = 1.67 kJ/mol) in the cell, given the typical intracellular concentrations of G6P and F6P are 0.0183 mM and 0.001mM, respectively [R = 8.314 J/K*mol, T= 310 K (37 ˚C)] (10 pts) 5) The next step in glycolysis is catalyzed by phosphofructokinase (PFK). The ΔG˚’ of this reaction is -14.2 kJ/mol. It is known that the ΔG˚’ of ATP hydrolysis reaction is -30.5 kJ/mol. Estimate the ΔG˚’ for the following reaction: Fructose-6-phosphate + phosphate ⇆ Fructose-1,6-bisphosphate (10 pts)