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Remember hilltopperitis = all those affected have blue hair…

Posted byAnonymous September 25, 2024March 6, 2025

Questions

Remember hilltоpperitis = аll thоse аffected hаve blue hair and yellоw skin, about 50% of those affected have tiny horns, and the others have very noticeable horns.  You are interested in finding the gene(s) responsible for the horn phenotype; you decide to approach this by using a microarray. You isolate mRNA from tiny horns and large horns. You use the mRNA to create fluorescently labeled cDNA -  blue (tiny) and yellow (large) (blue+yellow = brownish-grey).  You use these samples to probe a human microarray that contains sequences representing ALL human protein coding genes. Explain the rationale for using samples from individuals with tiny horns and large horns.  How will these samples be useful for identifying genes involved in this phenotype? (In other words, if a gene is responsible for the horn phenotype, how will it's expression be different in individuals with tiny horns and large horns?)

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