Sectiоn B - Histоlоgy You only need to аnswer one of the two questions from this section. An аnswer box will be beneаth this box - please indicate which question you are answering, for example: 'My Answer to Question X is...' Questions are as followed: Question 4: Answer both parts of the question below a) Describe the workflow for H&E staining for formalin-fixed paraffin-embedded slides, including their mechanisms of action and the molecules that they stain. (50%) b) The two pairs of images below show normal and pathological states. In each case, state what staining method has been used and the organ the section is from. Describe the clinical features detected by the staining method in the disease that allows for diagnosis. In each case the healthy tissue is in the left-hand panel. (50%) i) ii) Question 5: Below is an image of staining using immunohistochemistry for the HER2 protein as a marker for breast cancer, from two patients (A and B). Answer both parts of the following question. a) State which patient has breast cancer and why based on the results and state where the HER2 protein is located in the cell. (10%) b) Compare and contrast the various methodologies for performing immunohistochemistry. You do not need to discuss antigen retrieval as part of the answer. Diagrams are encouraged. (90%)