Dye terminator sequencing has been performed using capillary electrophoresis to separate the fragments.  ddATP has been labeled with a green fluor, ddTTp with red, ddCTP with blue and ddGTP with black.  The colors are detected in the following order:  red, green, black, red, green blue, red, black, black, green and blue.  What is the sequence of this fragment?

Which of the following is the output from capillary electrophoresis of a fluorescent product? 

A linear DNA molecule with one restriction enzyme recognition site will yield how many fragments when digested with that restriction enzyme?

A DNA polymorphism is a sequence difference compared to a reference standard that is present in at least 50% of the population.

The Southern blot transfer procedure includes:

Concisely explain how you would prepare a 1% agarose gel to visualize extracted DNA.  Be certain to include the essential components, how much of each you need, and the general process.   

What is the yield of DNA from a sample with a concentration of 280 ug/mL and a volume of 0.5 mL?

This is a closed book, closed note, closed browser quiz.  You can use a blank white board and a calculator on this quiz.  Any indication of academic dishonesty will be submitted to the GW Office of Academic Integrity.  You have one attempt to complete this quiz.

A plasmid has two sites for the restriction enzyme EcoR1 and one BamHI site.  How many fragments will result when you digest the plasmid with both EcoR1 and BamHI?

Which molecule polymerizes to form a support medium through which nucleic acids move?