You are a researcher working with recombinant DNA technology (e.g. moving DNA from one donor organism into an unrelated recipient organism) and you have two possible recipient strains to choose from. One strain has a mutation that knocks out the cell’s restriction systems and the other strain has a mutation that knocks out the cell’s modification systems.  (Neither of these damage the recipient cell’s ability to function). Which strain would you choose to introduce your recombinant DNA into and why, to increase the success of these DNA transfer experiments?

Extreme thermophilic DNA polymerases like Vent and DeepVent have decreased accuracy (fidelity) when compared to Taq polymerase because of their proofreading activity. 

Extreme thermophilic DNA polymerases like Vent and DeepVent have increased accuracy (fidelity) when compared to Taq polymerase because of their lack of a proofreading activity. 

Using the CRISPR-Cas system to genetically engineer organisms relies on our ability to make

Which of these is true about the primers used in a PCR reaction? 

You are developing an experiment to determining the quantity of DNA target molecules in a sample. Which of the three PCR techniques (“Regular” PCR, RT-PCR, qPCR) would be most appropriate for this?

If you think about the entire process of gene expression, from DNA to protein, you could argue that the actual step of “translation” is when

True/False regarding bacterial transcription. All of the promoter sequences are the same for all of the genes in any one species in the Domain Bacteria.

Comparing and contrasting RNA polymerase that makes RNA and DNA polymerase that makes DNA , identify which statements are true (accurate) and which are false (inaccurate). This is a multiple answer question. 

If the following piece of double-stranded DNA is transcribed, using the TOP strand as the template strand, what would be the RNA molecule produced?   5’TCCGCAACACTGT3′ 3’AGGCGTTGTGACA5′