After fixing the issue described above, you realize you neve…
After fixing the issue described above, you realize you never checked the pixel size and the resolution of your optical system. A glance at the manual and some quick calculations reveal that the pixel size of your camera is 25 µm and the optical resolution is 200nm for your chosen fluorophores. When imaging with 60x magnification, does your system meet the Nyquist criterion? Use scratch paper to explain your answer and show your calculations. Then use your cell phone to take a photo and submit the file below.
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