In the following question, match the filtercubes (excitation filter, dichroic mirror, emission filter) below to the most appropriate fluorophores. Filtercube 1:   Filtercube 2:   Fluorophore choices mCerulean3   B. mEGFP   C. mOrange2   D. PATagRFP

Which force measurement mode is most appropriate for measuring folding/unfolding rate constants of a molecule?

ATOMIC FORCE MICROSCOPY

Explain how positive peaks are formed by an endothermic reaction in the timetrace of output power in ITC.

Rank the following techniques in order of lowest resolution (greater d) to highest resolution (smaller d): SIM [SIM] PALM/STORM [PALMSTORM] STED [STED] TIRF [TIRF]

You are investigating the effect of a novel protein, HERKY3, on the interaction kinetics of the interaction between two other proteins, HAWKS and RULE. First, you run an experiment where HAWKS is immobilized on a chip and RULE is flowed in (solid line). Then, you repeat the experiment, but in the presence of HERKY3. The addition of HERKY3 has one effect: it causes the dissociation rate of HAWKS and RULE to decrease. Which sensorgram (dashed line) corresponds to this result when compared to HAWKS-RULE alone (solid line)? Note that all dashed lines are slightly raised from the solid lines to differentiate them visually.

IMAGING

Give two approaches that can be implemented to solve the mass transport limitation

Fill in the blanks: Phase contrast microscopy converts a difference in [variable1] to a difference in [variable2].

OK, what question would you have included on the exam that did not appear? Write your question and the answer you would have provided.