Discuss the complete procedure for conducting air- and bone-…
Discuss the complete procedure for conducting air- and bone-conduction pure-tone audiometry, including patient instructions, clinician responsibilities, and methods for threshold determination. Make sure you add information on each of these aspects.
Read DetailsYou use replica plating to isolate a culture of an E. coli a…
You use replica plating to isolate a culture of an E. coli auxotroph, unable to grow without tryptophan, from a culture of prototrophs. For a number of months, you propagate your tryptophan auxotroph culture on GSA (glucose salts agar) medium supplemented with tryptophan. One day, you mistakenly transfer some of your strain onto a new GSA plate that does not contain tryptophan. You realize your mistake after you have left the lab for the day and it is too late to correct it. However, when you return following 24 hours of incubation, you discover a few colonies of E. coli growing on the GSA medium. Please explain this result: why were you able to originally obtain a tryptophan auxotroph from a prototroph culture, and why months later did you find E. coli growth on GSA lacking tryptophan?
Read DetailsYou conduct an Ames test on a chemical you suspect might be…
You conduct an Ames test on a chemical you suspect might be a carcinogen. Following incubation, you count 3 colonies on your control plate (no chemical added) and over 400 colonies on your experimental plate (chemical added). From this information, you can conclude ______.
Read DetailsConsider the cyanobacteria Nostoc. Individual cells grow att…
Consider the cyanobacteria Nostoc. Individual cells grow attached to each other in long chains produced from binary fission with cells remaining attached (e.g., streptococcus). Most perform photosynthesis, using photosystems I and II to generate ATP and NADPH that they use to power the fixation of carbon dioxide via the Calvin cycle. However, a small number form heterocysts, cells that instead of performing photosynthesis, fix nitrogen into ammonium. Heterocysts share their nitrogen compounds with their photosynthetic neighbors, who in turn share sugars with the heterocysts. If you extracted and analyzed all the mRNA from a single heterocyst and all the mRNA from a single photosynthetic cell and compared both sets of mRNA, would you find mRNA that is different between these two cell types, photosynthetic and nitrogen fixing heterocyst? If so, what would it represent?
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