32.  The nurse estimates the extent of a burn using the rule of nines for a client who has been admitted with deep partial-thickness burns of the anterior right arm, entire left arm and anterior left leg. What percentage of the client’s total body surface area (TBSA) has been injured?  

Dr. Porter suspects that his patient’s decubitus ulcer may be caused by an anaerobic organism.  Explain processing to isolate the suspected organism.  Include how you would promote an anaerobic environment for organism growth, from specimen collection to incubation.  Also explain the following media used: SBA, BBE, KVLB and nutrient broth and how the media would promote growth.  List the medias’ selective ingredients (if present) and how they inhibit growth of some organisms and how organisms can be differentiated using the media.

Which gram negative bacilli might produce a black pigment and brick-red fluorescence when exposed to an UV light source?

Of the following, what disease does Clostridium perfringens cause?

An aspirate from a deep wound was plated on blood agar plates aerobically and anaerobically.  At 24 hours there was growth on both plates.  This indicates that the organism is

This level of the biofilm contains organisms that downregulate very efficiently and are the least metabolically active.

What are the metabolically inert microorganisms present in a biofilm called? 

What is the common name for a dental biofilm?

What test(s) can be used to presumptively identify/differentiate Peptostreptococcus species?

Biofilms are a survival strategy for all of the following EXCEPT