Discuss the complete procedure for conducting air- and bone-…
Discuss the complete procedure for conducting air- and bone-conduction pure-tone audiometry, including patient instructions, clinician responsibilities, and methods for threshold determination. Make sure you add information on each of these aspects.
Read DetailsYou use replica plating to isolate a culture of an E. coli a…
You use replica plating to isolate a culture of an E. coli auxotroph, unable to grow without tryptophan, from a culture of prototrophs. For a number of months, you propagate your tryptophan auxotroph culture on GSA (glucose salts agar) medium supplemented with tryptophan. One day, you mistakenly transfer some of your strain onto a new GSA plate that does not contain tryptophan. You realize your mistake after you have left the lab for the day and it is too late to correct it. However, when you return following 24 hours of incubation, you discover a few colonies of E. coli growing on the GSA medium. Please explain this result: why were you able to originally obtain a tryptophan auxotroph from a prototroph culture, and why months later did you find E. coli growth on GSA lacking tryptophan?
Read DetailsYou conduct an Ames test on a chemical you suspect might be…
You conduct an Ames test on a chemical you suspect might be a carcinogen. Following incubation, you count 3 colonies on your control plate (no chemical added) and over 400 colonies on your experimental plate (chemical added). From this information, you can conclude ______.
Read Details