You are performing a lineage tracing experiment to test whet…
You are performing a lineage tracing experiment to test whether any liver cells can migrate away form the liver organ. You decide to implement a genetic lineage tracing strategy (outlined below) to track liver cells throughout the life of your study organism. You establish two separate transgenic lines Line 1 and Line 2. Line1 has a transgene that drives Cre recombinase under the control of a strong liver enhancer plus promoter. Line 2 features a transgene that drives a green fluorescent protein (GFP) with a stop codon followed by a red fluorescent protein (RFP) gene under the control of a housekeeping enhancer plus promoter. The GFP portion of this transgene (including the stop codon) is flanked by LoxP sites, which upon Cre exposure, cause removal of the GFP coding region and leave the RFP coding region in-frame. Line 1: Line 2: Before we cross Line1 and Line2 together… we expect Line1 to demonstrate [blank1] fluorescence by itself. we expect Line2 to demonstrate [blank2] fluorescence by itself. After crossing Line1 and Line2 together… we expect brain cells to demonstrate [blank3] fluorescence. we expect liver cells to demonstrate [blank4] fluorescence. if you find cells that demonstrate red fluorescence outside of the liver, this would [blank5] your hypothesis.
Read DetailsNote: If you have been given prior permission to take the ex…
Note: If you have been given prior permission to take the exam remotely (illness, etc), don’t forget that you need to scan your room and show both sides of any scratch paper you have with you! Is the US income distribution getting wider? (1 point) Support your argument making reference to data seen in class; explain how those data are measured and how that measure of the income distribution is calculated. (1 point) Now present data that support a different point of view and explain what it is that is different about those data from the measure. Why do you prefer your measure to this second one? Based on the data seen in class, do you think that it is getting easier or harder to move up in the income distribution than it used to be? (1 point) Support your argument making reference to data seen in class; explain both how those data are measured and how that measure of mobility is calculated. (1 point) Now present data that support a different point of view and explain what it is that is different about those data from the measure. Why do you prefer your measure to this second one? Raj Chetty et al show that relocation can often improve the odds of upward mobility. (1 point) What is one locational factor (social capital) that Chetty et al show impacts economic mobility? What factors do they control for to establish this correlation? (1 point) What is a second locational factor (social capital) that Chetty et al show impacts economic mobility? What factors do they control for to establish this correlation?
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