You prepare bacterial cell extracts by lysing the cells and…
You prepare bacterial cell extracts by lysing the cells and removing insoluble debris via centrifugation. These extracts provide the proteins required for DNA replication. Your DNA template is a small, double-stranded circular piece of DNA (a plasmid, figure below) with a single origin of replication and a single replication termination site. The termination site is on the opposite side of the plasmid from the origin. Which of the following statements is TRUE with respect to this in vitro replication system?
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