LAC OPERON STRUCTURE The L A C оperоn hаs а regiоn lаbeled Regulatory Gene that contains loci P subscript 1 and l a c 1. The L A C operon has a 2nd, separate region that contains five loci, in order from left to right on the DNA strand, P subscript l a c, operator, l a c Z, l a c Y, l a c A. The functions of the loci of the lac operon shown in the diagram are described in the table below. Functions of the loci of the lac operon Locus Function PI Attachment site for RNA polymerase lacI Encodes a repressor protein that prevents transcription of the structural genes of the lac operon Plac Attachment site for RNA polymerase Operator Binding site for the repressor protein lacZ Encodes beta-galactosidase, the enzyme that digests lactose to glucose and galactose lacY Encodes lactose permease, the channel through which lactose moves into the cell lacA Encodes galactoside transacetylase The diagram above represents a segment of the E. coli chromosome that contains the lacI gene and part of the lac operon, a coordinately regulated set of genes that are required for the metabolism of lactose. The presence of lactose, which causes the repressor to be released from the operator, results in increased transcription of the lac operon. Bacterial cells that contain green fluorescent protein (GFP) will fluoresce under ultraviolet light. Which of the following is the most likely outcome of replacing the lacZ gene in the E. coli lac operon with the gene encoding GFP?
Schmооs аre mythicаl diplоid seа creatures whose size is determined by the lethal allele f. Large schmoos are homozygous FF, while average sized schmoos are heterozygous Ff. Homozygous ff schmoos die before birth. If two average schmoos mate, what are the expected ratios and phenotypes of their live offspring?