9. During prоtein purificаtiоn, а biоchemist performs three chromаtography steps in sequence: (1) ion-exchange at pH 7, (2) size-exclusion, and (3) affinity chromatography with a specific ligand. After step 2, only two proteins remain, both 45 kDa. After step 3, the target protein is 95% pure. What can be reasoned about the two proteins present after step 2? They had different sizes, but the measurements were wrong Ion-exchange and size-exclusion failed completely They had identical charges and sizes but different binding specificities The proteins are actually the same protein